Multiplexed Guide RNA Expression Leads to Increased Mutation Frequency in Targeted Window Using a CRISPR-Guided Error-Prone DNA Polymerase in Saccharomyces cerevisiae
- Michael Gossing, Angelo Limeta, Christos Skrekas, …, Andrew Davis, Verena Siewers, Florian David
- ACS Synth Biol, 10.1021/acssynbio.2c00689
Targeted In Vivo Mutagenesis in Yeast Using CRISPR/Cas9 and Hyperactive Cytidine and Adenine Deaminases
- Christos Skrekas, Angelo Limeta, Verena Siewers, …, Angelo Limeta, Verena Siewers, Florian David
- ACS Synth Biol, 10.1021/acssynbio.2c00690
Directed evolution of a wax ester synthase for production of fatty acid ethyl esters in Saccharomyces cerevisiae
- Juan Octavio Valle-RodrÃguez, Verena Siewers, Jens Nielsen, …, Verena Siewers, Jens Nielsen, Shuobo Shi
- Appl Microbiol Biotechnol, 10.1007/s00253-023-12466-8
Functional genome annotation and transcriptome analysis of Pseudozyma hubeiensis BOT-O, an oleaginous yeast that utilizes glucose and xylose at equal rates
- Friederike Mierke, Daniel P Brink, Joakim Norbeck, …, Joakim Norbeck, Verena Siewers, Thomas Andlid
- Fungal Genet Biol, 10.1016/j.fgb.2023.103783
Identification of acetic acid sensitive strains through biosensor-based screening of a Saccharomyces cerevisiae CRISPRi library
- Maurizio Mormino, Ibai Lenitz, Verena Siewers, Yvonne Nygård
- Microb Cell Fact, 10.1186/s12934-022-01938-7
Genetically Encoded Whole Cell Biosensor for Drug Discovery of HIF-1 Interaction Inhibitors
- Louis H Scott, Mark J Wigglesworth, Verena Siewers, …, Verena Siewers, Andrew M Davis, Florian David
- ACS Synth Biol, 10.1021/acssynbio.2c00274
A p -Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
- Dany Liu, Maria Sole Sica, Jiwei Mao, …, Jiwei Mao, Lucy Fang-I Chao, Verena Siewers
- ACS Synth Biol, 10.1021/acssynbio.2c00111
Controlling gene expression with deep generative design of regulatory DNA
- Jan Zrimec, Xiaozhi Fu, Azam Sheikh Muhammad, …, Florian David, Jens Nielsen, Aleksej Zelezniak
- Nat Commun, 10.1038/s41467-022-32818-8
Reconstruction of a catalogue of genome-scale metabolic models with enzymatic constraints using GECKO 2.0
- Iván Domenzain, BenjamÃn Sánchez, Mihail Anton, …, John P Morrissey, Nikolaus Sonnenschein, Jens Nielsen
- Nat Commun, 10.1038/s41467-022-31421-1
Identification of a novel gene required for competitive growth at high temperature in the thermotolerant yeast Kluyveromyces marxianus
- Noemi Montini, Tyler W Doughty, Iván Domenzain, …, Jens Nielsen, Verena Siewers, John P Morrissey
- Microbiology (Reading), 10.1099/mic.0.001148
A Hypersensitive Genetically Encoded Fluorescent Indicator (roGFP2-Prx1) Enables Continuous Measurement of Intracellular H 2 O 2 during Cell Micro-cultivation
- Veronica Gast, Verena Siewers, Mikael Molin, Veronica Gast, Verena Siewers, Mikael Molin
- None, 10.21769/bioprotoc.4317
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries
- Maximilian Otto, Christos Skrekas, Michael Gossing, Johan Gustafsson, Verena Siewers, Florian David
- ACS Synth Biol, 10.1021/acssynbio.1c00408
