RabLab May 2023

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Proceedings of the National Academy of Sciences of the United States of America

Impact of acute stress on murine metabolomics and metabolic flux.

2023-05-15

PubMed:37186827; DOI:10.1073/pnas.2301215120

Won Dong Lee¹, Lingfan Liang¹, Jenna AbuSalim², Connor S R Jankowski², Laith Z Samarah¹, Michael D Neinast¹, Joshua D Rabinowitz¹

Affiliations

Department of Chemistry, Princeton University, Princeton, NJ 08544
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544

What is the most stress-sensitive plasma metabolite?

Today @PNASNews, we show lactate as the most stress-sensitive metabolite in circulation – blood lactate level increases ~5-fold within seconds of stress or adrenergic activation.

2023-05-15

human-health

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Metabolites

Bone Metabolite Profile Differs between Normal and Femur Head Necrosis (FHN/BCO)-Affected Broilers: Implications for Dysregulated Metabolic Cascades in FHN Pathophysiology.

2023-05-16

PubMed:37233703; DOI:10.3390/metabo13050662

Alison Ramser¹, Rachel Hawken², Elizabeth Greene¹, Ron Okimoto², Brenda Flack², Courtney J Christopher³, Shawn R Campagna³, Sami Dridi¹

Affiliations

Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA
Cobb-Vantress, Siloam Springs, AR 72761, USA
Department of Chemistry, University of Tennessee, Knoxville, TN 37996, USA

Frontiers in cell and developmental biology

Metabolite abundance in bovine preovulatory follicular fluid is influenced by follicle developmental progression post estrous onset in cattle.

2023-05-05

PubMed:37215073; DOI:10.3389/fcell.2023.1156060

Emma A Hessock¹, J Lannett Edwards¹, F Neal Schrick¹, Rebecca R Payton¹, Shawn R Campagna², Abigayle B Pollock¹, Hannah M Clark¹, Allyson E Stokes¹, Jessica L Klabnik¹, Kennedy S Hill¹, Samantha R Roberts¹, Meredith G Hinson¹, Sarah E Moorey¹

Affiliations

Department of Animal Science, University of Tennessee, Knoxville, TN, United States
Department of Chemistry, University of Tennessee, Knoxville, TN, United States

New Research: Metabolite abundance in bovine preovulatory follicular fluid is influenced by follicle developmental progression post estrous onset in cattle: Introduction: Preovulatory follicle response to the luteinizing hormone (LH) surge… #celldevbio

2023-05-05

Klabnik Lab News: Collaborative work with Moorey Lab just published!

Metabolite abundance in bovine preovulatory follicular fluid is influenced by follicle developmental progression post estrous onset in cattle.

2023-05-08

Cell host & microbe

Four-part harmony between diet, drugs, microbes, and host.

2023-05-11

PubMed:37167950; DOI:10.1016/j.chom.2023.04.013

Patrick H Bradley¹

Affiliations

Department of Microbiology, The Ohio State University, Columbus, OH 43201, USA; Infectious Diseases Institute, The Ohio State University, Columbus, OH 43201, USA.

Four-part harmony between #diet, drugs, microbes, and host. @BradleyPH highlights work showing that a specific gut #microbe causes diet-dependent attenuation of acetaminophen toxicity in mice https://cell.com/cell-host-microbe/fulltext/S1931-3128(23)00164-6…

2023-05-10

EMBO reports

Ovol1/2 loss-induced epidermal defects elicit skin immune activation and alter global metabolism.

2023-05-30

PubMed:37249012; DOI:10.15252/embr.202256214

Morgan Dragan¹, Zeyu Chen¹, Yumei Li¹, Johnny Le¹, Peng Sun¹, Daniel Haensel¹, Suhas Sureshchandra², Anh Pham¹, Eddie Lu¹, Katherine Thanh Pham¹, Amandine Verlande¹, Remy Vu¹, Guadalupe Gutierrez¹, Wei Li¹, Cholsoon Jang¹, Selma Masri¹, Xing Dai¹

Affiliations

Department of Biological Chemistry, School of Medicine, University of California, Irvine, CA, USA
Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA

Nature communications

Genetics implicates overactive osteogenesis in the development of diffuse idiopathic skeletal hyperostosis.

2023-05-08

PubMed:37156767; DOI:10.1038/s41467-023-38279-x

Anurag Sethi¹, J Graham Ruby², Matthew A Veras², Natalie Telis², Eugene Melamud³

Affiliations

Calico Life Sciences, LLC, South San Francisco, CA, 94080, USA.
Calico Life Sciences, LLC, South San Francisco, CA, 94080, USA
Calico Life Sciences, LLC, South San Francisco, CA, 94080, USA.

Genetics implicates overactive osteogenesis in development of diffuse idiopathic skeletal hyperostosis. A Sethi et al. Nat Commun 2023 May 8
https://rb.gy/2c148 …largest analysis of DISH to-date with machine learning (lat. DXA and genetic analysis implicated 10 novel loci

2023-06-01

Genetics implicates overactive osteogenesis in the development of diffuse idiopathic skeletal hyperostosis

2023-05-10

Nature metabolism

Reply to: revisiting the role of serine metabolism in hepatic lipogenesis.

2023-05-11

PubMed:37169877; DOI:10.1038/s42255-023-00793-z

Zhaoyue Zhang¹, Tara TeSlaa², Joshua D Rabinowitz³

Affiliations

Department of Chemistry, Princeton University, Princeton, NJ, USA
Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA
Department of Chemistry, Princeton University, Princeton, NJ, USA.

And the reply led by Joshua D. Rabinowitz (@Princeton):

2023-06-09

American journal of physiology. Renal physiology

Renal histaminergic system and acute effects of histamine receptor 2 blockade on renal damage in the Dahl salt-sensitive rat.

2023-05-25

PubMed:37227223; DOI:10.1152/ajprenal.00269.2022

Denisha R Spires¹, Ryan S Schibalski¹, Mark Domondon², Callie Clarke², Samantha Perez², Fabiha Anwar¹, Emily Burns¹, Muhammad Irfan Saeed³, Samuel D Walton¹, Aleksandra S Zamaro¹, Thelma Amoah², Sergey N Arkhipov⁴, Courtney J Christopher⁵, Shawn R Campagna⁵, David L Mattson¹, Tengis S Pavlov⁴, Daria V Ilatovskaya¹

Affiliations

Department of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, United States
Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, United States
Augusta University Medical Center, Augusta, Georgia, United States
Hypertension and Vascular Research, Henry Ford Health, Detroit, Michigan, United States
Department of Chemistry, University of Tennessee, Knoxville, Tennessee, United States

Hot off the press in @AJPRenal : Renal Histaminergic System, with @spi_dr as first author and outstanding help from @CampagnaCommand @CardiorenalRese, @ryanschibalski @EmilyCBurns and many others! https://tinyurl.com/m6we2uen

2023-05-31

Congrats Courtney on the publication with the Ilatovskaya Lab at Augusta University!
@AUG_University
@CHEMISTRYUTK
@UTKnoxville

2023-05-30

Frontiers in aging

Sex differences in pharmacological interventions and their effects on lifespan and healthspan outcomes: a systematic review.

2023-05-22

PubMed:37305228; DOI:10.3389/fragi.2023.1172789

Marie Knufinke¹, Michael R MacArthur¹, Collin Y Ewald¹, Sarah J Mitchell¹

Affiliations

Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland

Sex differences in pharmacological interventions and their effects on lifespan and healthspan outcomes: a systematic review

Marie Knufinke, @MikeMacArthur8, @CollinEwald, @sarahmitchelphd
https://frontiersin.org/articles/10.3389/fragi.2023.1172789/full…
@FrontAging

Fig 2 (L) Studies included.
Fig 3 (R) ” Since 2020.

2023-06-13

Strikingly, 73% of studies of current #longevity #drugs showed #sex-specific outcomes on #healthspan & lifespan in #preclinical models. @MarieKnufinke, @MikeMacArthur8, @sarahmitchelphd
https://doi.org/10.3389/fragi.2023.1172789…

2023-06-02

eLife

Osteoblast-intrinsic defect in glucose metabolism impairs bone formation in type II diabetic male mice.

2023-05-05

PubMed:37144869; DOI:10.7554/eLife.85714

Fangfang Song¹, Won Dong Lee², Tyler Marmo¹, Xing Ji¹, Chao Song¹, Xueyang Liao¹, Rebecca Seeley¹, Lutian Yao¹, Haoran Liu³, Fanxin Long¹

Affiliations

Translational Research Program in Pediatric Orthopedics, Department of Surgery, The Children’s Hospital of Philadelphia, Philadelphia, United States
Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, United States
Department of Computer Science, New Jersey Institute of Technology, Newark, United States

How can we restore bone formation and prevent skeletal fragility in type 2 diabetes?

We demonstrated in a youth-onset T2D mouse model that activation of glycolysis in osteoblasts can do so.

https://doi.org/10.7554/eLife.85714… @eLife

2023-05-19

bioRxiv

IL-6 is dispensable for causing cachexia in the colon carcinoma 26 model

2023-05-02

DOI:10.1101/2023.05.02.539076

Y.-Y. Kwon, S. Hui

Affiliations

Happy to share our work on cancer cachexia. Cytokines occupy more than half of the list of proposed cachectic factors. One prominent example is IL-6, which has been deemed a key cachectic factor in the C26 mouse model, one of the most widely used models of cancer cachexia.

2023-05-03

bioRxiv

Cytomegalovirus-induced inactivation of TSC2 disrupts the coupling of fatty acid biosynthesis to glucose availability resulting in a vulnerability to glucose limitation

2023-05-19

DOI:10.1101/2023.05.17.541212

M. H. Raymonda, I. Rodriguez-Sanchez, X. Schafer, L. Smorodintsev-Schiller, I. S. Harris, J. Munger

Affiliations

University of Rochester Medical Center

bioRxiv

Ablation of Sam50 is associated with fragmentation and alterations in metabolism in human myotubes

2023-05-22

DOI:10.1101/2023.05.20.541602

Z. Vue, C. Vang, K. Neikirk, E. Garza-Lopez, J.-q. Shao, M. Mungai, L. Vang, H. K. Beasley, A. G. Marshall, A. Crabtree, D. C. Stephens, M. McReynolds, S. A. Murray, P. Katti, J. A. Gaddy, S. M. Damo, A. Hinton

Affiliations

Vanderbilt University

Preprint alert
From @AtHinton lab and collaborators including @stevendamolab1: “Ablation of Sam50 is associated with fragmentation and alterations in metabolism in human myotubes”

https://biorxiv.org/content/10.1101/2023.05.20.541602v1…
@VUBasicSciences @Fisk1866

2023-05-31

bioRxiv

FAM210A Regulates Mitochondrial Translation and Maintains Cardiac Mitochondrial Homeostasis

2023-05-22

DOI:10.1101/2023.05.20.541585

J. Wu, K. C. V. Subbaiah, O. Hedaya, S. Chen, J. Munger, W. H. W. Tang, C. Yan, P. Yao

Affiliations

Aab Cardiovascular Research Institute, Department of Medicine, Department of Biochemistry & Biophysics, University of Rochester School of Medicine & Dentistry,

FAM210A Regulates Mitochondrial Translation and Maintains Cardiac Mitochondrial Homeostasis

2023-05-27

other

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Nature communications

Optimal enzyme utilization suggests that concentrations and thermodynamics determine binding mechanisms and enzyme saturations.

2023-05-05

PubMed:37147292; DOI:10.1038/s41467-023-38159-4

Asli Sahin¹, Daniel R Weilandt¹, Vassily Hatzimanikatis²

Affiliations

Laboratory of Computational Systems Biotechnology, Ecole Polytechnique Federale de Lausanne (EPFL), 1015, Lausanne, Switzerland
Laboratory of Computational Systems Biotechnology, Ecole Polytechnique Federale de Lausanne (EPFL), 1015, Lausanne, Switzerland.

Goodmorning 🙂 Excited that our latest work (https://nature.com/articles/s41467-023-38159-4…) just got published at
@NatureComms! In this work, we introduce a framework to explore the catalytically optimal operating conditions of any complex enzyme mechanism from an evolutionary perspective.

2023-05-08

Ever thought about how evolution shapes enzyme binding mechanisms?
Exited to share this work of @aslisahin2205 @realLCSB with @Vassily_13. We show that cellular concentrations and thermodynamics determine the optimal binding sequence and enzyme saturation.

2023-05-08

Beyond happy that our latest work is now available at
@NatureComms!
In this work, we present an evolutionary approach to enzyme kinetics and introduce our computational framework to explore the catalytically optimal enzyme.

2023-05-08

cancer-and-disease

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Molecular carcinogenesis

Histone deacetylase inhibitor belinostat regulates metabolic reprogramming in killing KRAS-mutant human lung cancer cells.

2023-05-05

PubMed:37144836; DOI:10.1002/mc.23551

Rebecca M Peter¹, Md Shahid Sarwar², Sarah Z Mostafa², Yujue Wang³, Xiaoyang Su³, Ah-Ng Kong²

Affiliations

Graduate Program in Pharmaceutical Science, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA
Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA
Metabolomics Shared Resource, Rutgers Cancer Institute of New Jersey, New Brunswick, New Jersey, USA

bioRxiv

Serine synthesis pathway upregulated by E-cadherin is essential for the proliferation and metastasis of breast cancers

2023-05-24

DOI:10.1101/2023.05.24.541452

G. Lee, C. Wong, A. Cho, J. J. West, A. J. Crawford, G. C. Russo, B. R. Si, J. Kim, L. Hoffner, C. Jang, M. Jung, R. D. Leone, K. Konstantopoulos, A. J. Ewald, D. Wirtz, S. Jeong

Affiliations

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA

microbes

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Analytical and bioanalytical chemistry

Dual stable isotopes enhance lipidomic studies in bacterial model organism Enterococcus faecalis.

2023-05-19

PubMed:37204445; DOI:10.1007/s00216-023-04750-3

Brittni Woodall¹, Elizabeth M Fozo², Shawn R Campagna³

Affiliations

Department of Chemistry, University of Tennessee, Knoxville, TN, USA
Department of Microbiology, University of Tennessee, Knoxville, TN, USA
Department of Chemistry, University of Tennessee, Knoxville, TN, USA.

So proud to finally have chapter 3 of my dissertation published! A true labor of love, but so happy with the end result. If you love mass spectrometry, and lipids, take a peek!

2023-05-23

Super excited to see this published! Excellent work with @CampagnaCommand and @BrittniWoodall #lipidsrule!
https://rdcu.be/dcONn

2023-05-23

Congratulations Brittni on the newest Campagna Lab and @fozolab publication in @AnalBioanalChem
https://rdcu.be/dcONn

2023-05-23

kedalionlabs.com

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