Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing
- Johan Blatt Rojek, Yogesh Basavaraju, Saranya Nallapareddy, …, Lise M Grav, Steffen Goletz, Lasse E Pedersen
- Biotechnol Bioeng, 10.1002/bit.28367
Immobilization-Free Binding and Affinity Characterization of Higher Order Bispecific Antibody Complexes Using Size-Based Microfluidics
- Andreas V Madsen, Oscar Mejias-Gomez, Lasse E Pedersen, Kerstin Skovgaard, Peter Kristensen, Steffen Goletz
- Anal Chem, 10.1021/acs.analchem.2c02705
Erratum: An optimized genome-wide, virus-free CRISPR screen for mammalian cells
- Kai Xiong, Karen Julie la Cour Karottki, Hooman Hefzi, …, Nathan E Lewis, Helene Faustrup Kildegaard, Lasse Ebdrup Pedersen
- Cell Rep Methods, 10.1016/j.crmeth.2021.100115
A dual-reporter system for investigating and optimizing protein translation and folding in E. coli
- Ariane Zutz, Louise Hamborg, Lasse Ebdrup Pedersen, …, Kaare Teilum, Kresten Lindorff-Larsen, Alex Toftgaard Nielsen
- Nat Commun, 10.1038/s41467-021-26337-1
Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli
- Louise Hamborg, Daniele Granata, Johan G Olsen, …, Alex Toftgaard Nielsen, Kresten Lindorff-Larsen, Kaare Teilum
- Commun Biol, 10.1038/s42003-021-02490-7
A synthetic RNA-mediated evolution system in yeast
- Emil D Jensen, Marcos Laloux, Beata J Lehka, …, Tadas Jakočiūnas, Michael K Jensen, Jay D Keasling
- Nucleic Acids Res, 10.1093/nar/gkab472
A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes
- Karen Julie la Cour Karottki, Hooman Hefzi, Songyuan Li, …, Gyun Min Lee, Helene Faustrup Kildegaard, Nathan E Lewis
- Metab Eng, 10.1016/j.ymben.2021.03.017
Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells
- Seunghyeon Shin, Su Hyun Kim, Sung Wook Shin, …, Lasse Ebdrup Pedersen, Jae Seong Lee, Gyun Min Lee
- ACS Synth Biol, 10.1021/acssynbio.0c00097
Multiplex secretome engineering enhances recombinant protein production and purity
- Stefan Kol, Daniel Ley, Tune Wulff, …, Helene Faustrup Kildegaard, Gyun Min Lee, Nathan E Lewis
- Nat Commun, 10.1038/s41467-020-15866-w
Genome-Wide CRISPRi-Based Identification of Targets for Decoupling Growth from Production
- Songyuan Li, Christian Bille Jendresen, Jenny Landberg, …, Nikolaus Sonnenschein, Sheila Ingemann Jensen, Alex Toftgaard Nielsen
- ACS Synth Biol, 10.1021/acssynbio.9b00143