Video highlight
Multiplexed Guide RNA Expression Leads to Increased Mutation Frequency in Targeted Window Using a CRISPR-Guided Error-Prone DNA Polymerase in Saccharomyces cerevisiae
- Michael Gossing, Angelo Limeta, Christos Skrekas, …, Andrew Davis, Verena Siewers, Florian David
- ACS Synth Biol, 10.1021/acssynbio.2c00689
Targeted In Vivo Mutagenesis in Yeast Using CRISPR/Cas9 and Hyperactive Cytidine and Adenine Deaminases
- Christos Skrekas, Angelo Limeta, Verena Siewers, …, Angelo Limeta, Verena Siewers, Florian David
- ACS Synth Biol, 10.1021/acssynbio.2c00690
Genetically Encoded Whole Cell Biosensor for Drug Discovery of HIF-1 Interaction Inhibitors
- Louis H Scott, Mark J Wigglesworth, Verena Siewers, …, Verena Siewers, Andrew M Davis, Florian David
- ACS Synth Biol, 10.1021/acssynbio.2c00274
Controlling gene expression with deep generative design of regulatory DNA
- Jan Zrimec, Xiaozhi Fu, Azam Sheikh Muhammad, …, Florian David, Jens Nielsen, Aleksej Zelezniak
- Nat Commun, 10.1038/s41467-022-32818-8
Fluorescence-Activated Cell Sorting as a Tool for Recombinant Strain Screening
- Christos Skrekas, Raphael Ferreira, Florian David
- Methods Mol Biol, 10.1007/978-1-0716-2399-2_4
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries
- Maximilian Otto, Christos Skrekas, Michael Gossing, Johan Gustafsson, Verena Siewers, Florian David
- ACS Synth Biol, 10.1021/acssynbio.1c00408
Rational gRNA design based on transcription factor binding data
- David Bergenholm, Yasaman Dabirian, Raphael Ferreira, Verena Siewers, Florian David, Jens Nielsen
- None, 10.1093/synbio/ysab014
Does co-expression of Yarrowia lipolytica genes encoding Yas1p, Yas2p and Yas3p make a potential alkane-responsive biosensor in Saccharomyces cerevisiae?
- Yasaman Dabirian, Christos Skrekas, Florian David, Verena Siewers
- PLoS One, 10.1371/journal.pone.0239882
Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid
- Maximilian Otto, Paulo Gonçalves Teixeira, Maria Isabel Vizcaino, Florian David, Verena Siewers
- Microb Cell Fact, 10.1186/s12934-019-1257-z
Model-Assisted Fine-Tuning of Central Carbon Metabolism in Yeast through dCas9-Based Regulation
- Raphael Ferreira, Christos Skrekas, Alex Hedin, …, Verena Siewers, Jens Nielsen, Florian David
- ACS Synth Biol, 10.1021/acssynbio.9b00258
Expanding the Dynamic Range of a Transcription Factor-Based Biosensor in Saccharomyces cerevisiae
- Yasaman Dabirian, Xiaowei Li, Yun Chen, Florian David, Jens Nielsen, Verena Siewers
- ACS Synth Biol, 10.1021/acssynbio.9b00144
FadR-Based Biosensor-Assisted Screening for Genes Enhancing Fatty Acyl-CoA Pools in Saccharomyces cerevisiae
- Yasaman Dabirian, Paulo Gonçalves Teixeira, Jens Nielsen, Verena Siewers, Florian David
- ACS Synth Biol, 10.1021/acssynbio.9b00118
Increasing jojoba-like wax ester production in Saccharomyces cerevisiae by enhancing very long-chain, monounsaturated fatty acid synthesis
- Leonie Wenning, Christer S Ejsing, Florian David, Richard R Sprenger, Jens Nielsen, Verena Siewers
- Microb Cell Fact, 10.1186/s12934-019-1098-9