Channel: Ribosome profiling

Inspired by: Edward Wallace

Ribosome profiling, or Ribo-Seq (also named ribosome footprinting), is an adaptation of a technique developed by Joan Steitz and Marilyn Kozak almost 50 years ago that Nicholas Ingolia and Jonathan Weissman adapted to work with next generation sequencing that uses specialized messenger RNA (mRNA) sequencing to determine which mRNAs are being actively translated. Wikipedia (CC BY-SA 3.0)

2023 Articles

7 518 articles

Protocol for analyzing intact mRNA poly(A) tail length using nanopore direct RNA sequencing

  • Koichi Ogami, Yuka Oishi, Shin-Ichi Hoshino, Koichi Ogami, Yuka Oishi, Shin-Ichi Hoshino
  • STAR Protoc, 10.1016/j.xpro.2023.102340
Comment Section

Ribosome Profiling in the Model Diatom Thalassiosira pseudonana

  • Monica Pichler, Andreas Meindl, Markus Romberger, …, Gunter Meister, Jan Medenbach, Thomas Mock
  • Curr Protoc, 10.1002/cpz1.843
Comment Section

Rewriting the message: Harnessing RNA pseudouridylation to tackle disease

  • Matias Montes, Nicole M Martínez, Matias Montes, Nicole M Martínez, Matias Montes, Nicole M Martínez
  • Mol Cell, 10.1016/j.molcel.2023.02.001
Comment Section

choros: correction of sequence-based biases for accurate quantification of ribosome profiling data

  • Amanda Mok, Robert Tunney, Gonzalo Benegas, …, Gonzalo Benegas, Edward W J Wallace, Liana F Lareau
  • bioRxiv : the preprint server for biology, 10.1101/2023.02.21.529452
Comment Section

Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification

  • Hirotatsu Imai, Daisuke Utsumi, Hidetsugu Torihara, …, Kenzo Takahashi, Hidehito Kuroyanagi, Akio Yamashita
  • Nucleic Acids Res, 10.1093/nar/gkad545
Comment Section

Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seq

  • Isabel Cristina Vélez-Bermúdez, Shu-Jen Chou, Ai-Ping Chen, …, Ai-Ping Chen, Wen-Dar Lin, Wolfgang Schmidt
  • STAR Protoc, 10.1016/j.xpro.2023.102520
Comment Section

Translational regulation by uORFs and start codon selection stringency

  • Thomas E Dever, Ivaylo P Ivanov, Alan G Hinnebusch, …, Thomas E Dever, Ivaylo P Ivanov, Alan G Hinnebusch
  • Genes Dev, 10.1101/gad.350752.123
Comment Section

Flow-Seq Method: Features and Application in Bacterial Translation Studies

Comment Section

uORF4u: a tool for annotation of conserved upstream open reading frames

  • Artyom A Egorov, Gemma C Atkinson, Artyom A Egorov, …, Gemma C Atkinson, Artyom A Egorov, Gemma C Atkinson
  • Bioinformatics, 10.1093/bioinformatics/btad323
Comment Section

Contributions of Ccr4 and Gcn2 to the Translational Response of C. neoformans to Host-Relevant Stressors and Integrated Stress Response Induction

  • Corey M Knowles, David Goich, Amanda L M Bloom, …, Amanda L M Bloom, Murat C Kalem, John C Panepinto
  • mBio, 10.1128/mbio.00196-23
Comment Section

2022 Articles

683 articles

BID-seq: The Quantitative and Base-Resolution Sequencing Method for RNA Pseudouridine

Comment Section

Thousands of human non-AUG extended proteoforms lack evidence of evolutionary selection among mammals

  • Stephen J Kiniry, Dmitry E Andreev, Jonathan M Mudge, …, Dmitry E Andreev, Jonathan M Mudge, Pavel V Baranov
  • Nat Commun, 10.1038/s41467-022-35595-6
Comment Section

Specific mechanisms of translation initiation in higher eukaryotes: the eIF4G2 story

  • Ekaterina D Shestakova, Victoria V Smirnova, Ivan N Shatsky, …, Victoria V Smirnova, Ivan N Shatsky, Ilya M Terenin
  • RNA, 10.1261/rna.079462.122
Comment Section

Discovering misannotated lncRNAs using deep learning training dynamics

Comment Section

Initiation at AUGUG and GUGUG sequences can lead to translation of overlapping reading frames in E. coli

  • Maximilian P Kohl, Maria Kompatscher, Nina Clementi, …, Nina Clementi, Lena Holl, Matthias D Erlacher
  • Nucleic Acids Res, 10.1093/nar/gkac1175
Comment Section

Transcriptome-wide analysis of pseudouridylation in Drosophila melanogaster

  • Wan Song, Ram Podicheti, Douglas B Rusch, …, Ram Podicheti, Douglas B Rusch, William Daniel Tracey
  • G3 (Bethesda), 10.1093/g3journal/jkac333
Comment Section

Deciphering the role of RNA structure in translation efficiency

  • Jianan Lin, Yang Chen, Yuping Zhang, …, Yuping Zhang, Haifan Lin, Zhengqing Ouyang
  • BMC Bioinformatics, 10.1186/s12859-022-05037-7
Comment Section

Eukaryotic translation initiation factor eIF4G2 opens novel paths for protein synthesis in development, apoptosis and cell differentiation

  • Yudi Liu, Jiuwei Cui, Andrew R Hoffman, …, Jiuwei Cui, Andrew R Hoffman, Ji-Fan Hu
  • Cell Prolif, 10.1111/cpr.13367
Comment Section

Distance-dependent inhibition of translation initiation by downstream out-of-frame AUGs is consistent with a Brownian ratchet process of ribosome scanning

Comment Section

The Emerging Role of uORF-Encoded uPeptides and HLA uLigands in Cellular and Tumor Biology

Comment Section