A balancing act: sex selection after pre-implantation genetic testing for aneuploidy for first versus second baby
- M J Bayefsky, J Shaw, D Hamer, …, R Martel, J Reich, J K Blakemore
- Hum Reprod, 10.1093/humrep/dead101
GlycoCAP: A Cell-Free, Bacterial Glycosylation Platform for Building Clickable Azido-Sialoglycoproteins
- Ariel Helms Thames, Sam J Moons, Derek A Wong, …, Thomas J Boltje, Bruce S Bochner, Michael C Jewett
- ACS Synth Biol, 10.1021/acssynbio.3c00017
A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenic Escherichia coli infections in mice
- Asher J Williams, Katherine F Warfel, Primit Desai, …, Michael C Jewett, Yung-Fu Chang, Matthew P DeLisa
- None, 10.3389/fmolb.2023.1085887
Cell Extracts from Bacteria and Yeast Retain Metabolic Activity after Extended Storage and Repeated Thawing
- Blake J Rasor, Ashty S Karim, Hal S Alper, …, Ashty S Karim, Hal S Alper, Michael C Jewett
- ACS Synth Biol, 10.1021/acssynbio.2c00685
Community science designed ribosomes with beneficial phenotypes
- Antje Krüger, Andrew M Watkins, Roger Wellington-Oguri, …, Anne E d’Aquino, Rhiju Das, Michael C Jewett
- Nat Commun, 10.1038/s41467-023-35827-3
Cell-free Biosynthesis of Peptidomimetics
- Kanghun Lee, Jessica A Willi, Namjin Cho, …, Inseon Kim, Michael C Jewett, Joongoo Lee
- None, 10.1007/s12257-022-0268-5
At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring
- Kirsten J Jung, Blake J Rasor, Grant A Rybnicky, …, Ashty S Karim, Julius B Lucks, Michael C Jewett
- None, 10.1101/2023.01.09.523248
Computationally-guided design and selection of high performing ribosomal active site mutants
- Camila Kofman, Andrew M Watkins, Do Soon Kim, …, Ashty S Karim, Rhiju Das, Michael C Jewett
- Nucleic Acids Res, 10.1093/nar/gkac1036
Cell-Free Protein Synthesis of Particulate Methane Monooxygenase into Nanodiscs
- Christopher W Koo, Jasmine M Hershewe, Michael C Jewett, …, Jasmine M Hershewe, Michael C Jewett, Amy C Rosenzweig
- ACS Synth Biol, 10.1021/acssynbio.2c00366
Ribosome Stalling of N -Linked Glycoproteins in Cell-Free Extracts
- Sean S Chung, Erik J Bidstrup, Jasmine M Hershewe, …, Katherine F Warfel, Michael C Jewett, Matthew P DeLisa
- ACS Synth Biol, 10.1021/acssynbio.2c00311
A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans
- Thapakorn Jaroentomeechai, Yong Hyun Kwon, Yiwen Liu, …, Michael C Jewett, Dario Mizrachi, Matthew P DeLisa
- Nat Commun, 10.1038/s41467-022-34029-7
Ribosome-mediated biosynthesis of pyridazinone oligomers in vitro
- Joongoo Lee, Jaime N Coronado, Namjin Cho, …, Andrew D Ellington, Eric V Anslyn, Michael C Jewett
- Nat Commun, 10.1038/s41467-022-33701-2
Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line
- Mathias I Nielsen, Noortje de Haan, Weston Kightlinger, …, Ieva Bagdonaite, Sergey Y Vakhrushev, Hans H Wandall
- Nat Commun, 10.1038/s41467-022-33806-8
Three-dimensional structure-guided evolution of a ribosome with tethered subunits
- Do Soon Kim, Andrew Watkins, Erik Bidstrup, …, Emily Roney, Rhiju Das, Michael C Jewett
- Nat Chem Biol, 10.1038/s41589-022-01064-w
Linking the Salmonella enterica 1,2-Propanediol Utilization Bacterial Microcompartment Shell to the Enzymatic Core via the Shell Protein PduB
- Nolan W Kennedy, Carolyn E Mills, Charlotte H Abrahamson, …, Michael C Jewett, Niall M Mangan, Danielle Tullman-Ercek
- J Bacteriol, 10.1128/jb.00576-21
Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom
- Grant A Rybnicky, Radeen A Dixon, Robert M Kuhn, Ashty S Karim, Michael C Jewett
- ACS Synth Biol, 10.1021/acssynbio.1c00503
Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping
- Blake J Rasor, Bastian Vögeli, Michael C Jewett, Ashty S Karim
- Methods Mol Biol, 10.1007/978-1-0716-1998-8_12
A Primer on Emerging Field-Deployable Synthetic Biology Tools for Global Water Quality Monitoring
- Walter Thavarajah, Matthew S Verosloff, Jaeyoung K Jung, …, Michael C Jewett, Sera L Young, Julius B Lucks
- None, 10.1038/s41545-020-0064-8
Analysis of the Innovation Trend in Cell-Free Synthetic Biology
- Conary Meyer, Yusuke Nakamura, Blake J Rasor, Ashty S Karim, Michael C Jewett, Cheemeng Tan
- None, 10.3390/life11060551
Engineering molecular translation systems
- Camila Kofman, Joongoo Lee, Michael C Jewett
- Cell Syst, 10.1016/j.cels.2021.04.001
Protein engineering for natural product biosynthesis and synthetic biology applications
- Miles A Calzini, Alexandra A Malico, Melissa M Mitchler, Gavin J Williams
- Protein Eng Des Sel, 10.1093/protein/gzab015
Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro
- Joongoo Lee, Kevin J Schwarz, Hao Yu, …, Andrew D Ellington, Jeffrey S Moore, Michael C Jewett
- Chem Commun (Camb), 10.1039/d0cc07740b
Toward sustainable, cell-free biomanufacturing
- Blake J Rasor, Bastian Vögeli, Grant M Landwehr, Jonathan W Bogart, Ashty S Karim, Michael C Jewett
- Curr Opin Biotechnol, 10.1016/j.copbio.2020.12.012
Characterizing and Controlling Nanoscale Self-Assembly of Suckerin-12
- Jasmine M Hershewe, William D Wiseman, James E Kath, …, Patrick B Dennis, Rajesh R Naik, Michael C Jewett
- ACS Synth Biol, 10.1021/acssynbio.0c00442
Cell-free systems for accelerating glycoprotein expression and biomanufacturing
- Jasmine Hershewe, Weston Kightlinger, Michael C Jewett
- J Ind Microbiol Biotechnol, 10.1007/s10295-020-02321-4
Principles Learned from the International Race to Develop a Safe and Effective COVID-19 Vaccine
- Ariel H Thames, Kristy L Wolniak, Samuel I Stupp, …, Kristy L Wolniak, Samuel I Stupp, Michael C Jewett
- None, 10.1021/acscentsci.0c00644
Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
- Joongoo Lee, Kevin J Schwarz, Do Soon Kim, Jeffrey S Moore, Michael C Jewett
- Nat Commun, 10.1038/s41467-020-18001-x
Synthetic Glycobiology: Parts, Systems, and Applications
- Weston Kightlinger, Katherine F Warfel, Matthew P DeLisa, Michael C Jewett
- ACS Synth Biol, 10.1021/acssynbio.0c00210
The importance and future of biochemical engineering
- Timothy A Whitehead, Scott Banta, William E Bentley, …, Steffen Schaffer, Laura Segatori, Ian Wheeldon
- Biotechnol Bioeng, 10.1002/bit.27364
A fully orthogonal system for protein synthesis in bacterial cells
- Nikolay A Aleksashin, Teresa Szal, Anne E d’Aquino, Michael C Jewett, Nora Vázquez-Laslop, Alexander S Mankin
- Nat Commun, 10.1038/s41467-020-15756-1
Ribosomes read new languages
- Michael C Jewett, Michael J Hammerling
- Nat Chem Biol, 10.1038/s41589-020-0522-0
De novo design of protein logic gates
- Zibo Chen, Ryan D Kibler, Andrew Hunt, …, Michael C Jewett, Scott E Boyken, David Baker
- Science, 10.1126/science.aay2790
Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin
- Lei Zhuang, Shuhui Huang, Wan-Qiu Liu, Ashty S Karim, Michael C Jewett, Jian Li
- Metab Eng, 10.1016/j.ymben.2020.03.009
Apparent size and morphology of bacterial microcompartments varies with technique
- Nolan W Kennedy, Jasmine M Hershewe, Taylor M Nichols, …, Carolyn E Mills, Michael C Jewett, Danielle Tullman-Ercek
- PLoS One, 10.1371/journal.pone.0226395
Sequential Glycosylation of Proteins with Substrate-Specific N -Glycosyltransferases
- Liang Lin, Weston Kightlinger, Sunaina Kiran Prabhu, …, Lai-Xi Wang, Michael C Jewett, Milan Mrksich
- None, 10.1021/acscentsci.9b00021
In vitro ribosome synthesis and evolution through ribosome display
- Michael J Hammerling, Brian R Fritz, Danielle J Yoesep, Do Soon Kim, Erik D Carlson, Michael C Jewett
- Nat Commun, 10.1038/s41467-020-14705-2
Design and Optimization of a Cell-Free Atrazine Biosensor
- Adam D Silverman, Umut Akova, Khalid K Alam, Michael C Jewett, Julius B Lucks
- ACS Synth Biol, 10.1021/acssynbio.9b00388
Mutational characterization and mapping of the 70S ribosome active site
- Anne E d’Aquino, Tasfia Azim, Nikolay A Aleksashin, Adam J Hockenberry, Antje Krüger, Michael C Jewett
- Nucleic Acids Res, 10.1093/nar/gkaa001
A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds
- Jennifer E Kay, Michael C Jewett
- Metab Eng, 10.1016/j.mec.2019.e00114
High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS
- José-Marc Techner, Weston Kightlinger, Liang Lin, …, Matthew P DeLisa, Michael C Jewett, Milan Mrksich
- Anal Chem, 10.1021/acs.analchem.9b04334
Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor
- Walter Thavarajah, Adam D Silverman, Matthew S Verosloff, Nancy Kelley-Loughnane, Michael C Jewett, Julius B Lucks
- ACS Synth Biol, 10.1021/acssynbio.9b00347
Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid
- Xiangyang Liu, Adam D Silverman, Khalid K Alam, …, Julius B Lucks, Michael C Jewett, Srivatsan Raman
- ACS Synth Biol, 10.1021/acssynbio.9b00348
Cell-free gene expression: an expanded repertoire of applications
- Adam D Silverman, Ashty S Karim, Michael C Jewett
- Nat Rev Genet, 10.1038/s41576-019-0186-3
Strategies for in vitro engineering of the translation machinery
- Michael J Hammerling, Antje Krüger, Michael C Jewett
- Nucleic Acids Res, 10.1093/nar/gkz1011
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
- Weston Kightlinger, Katherine E Duncker, Ashvita Ramesh, …, Milan Mrksich, Matthew P DeLisa, Michael C Jewett
- Nat Commun, 10.1038/s41467-019-12024-9
A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli
- Benjamin J Des Soye, Vincent R Gerbasi, Paul M Thomas, Neil L Kelleher, Michael C Jewett
- Cell Chem Biol, 10.1016/j.chembiol.2019.10.008
Expanding the limits of the second genetic code with ribozymes
- Joongoo Lee, Kenneth E Schwieter, Andrew M Watkins, …, Andrew D Ellington, Jeffrey S Moore, Michael C Jewett
- Nat Commun, 10.1038/s41467-019-12916-w
Point-of-care biomarker quantification enabled by sample-specific calibration
- Monica P McNerney, Yan Zhang, Paige Steppe, Adam D Silverman, Michael C Jewett, Mark P Styczynski
- Sci Adv, 10.1126/sciadv.aax4473