
Gene expression dynamics in input-responsive engineered living materials programmed for bioproduction
- Hal S Alper
- James Carothers
- June 5, 2023
- kedalionlabs.com
- Widianti Sugianto, Gokce Altin-Yavuzarslan, Benjamin I Tickman, …, Hal S Alper, Alshakim Nelson, James M Carothers
- None, 10.1016/j.mtbio.2023.100677
Standards, dissemination, and best practices in systems biology
- James Carothers
- April 2, 2023
- kedalionlabs.com
- Janis Shin, Veronica Porubsky, James Carothers, …, Veronica Porubsky, James Carothers, Herbert M Sauro
- Curr Opin Biotechnol, 10.1016/j.copbio.2023.102922
Perspectives for self-driving labs in synthetic biology
- Blake Alexander Simmons
- Héctor GarcÃa MartÃn
- James Carothers
- January 5, 2023
- kedalionlabs.com
- Hector G Martin, Tijana Radivojevic, Jeremy Zucker, …, Deepti Tanjore, Lavanya Ramakrishnan, Anup Singh
- Curr Opin Biotechnol, 10.1016/j.copbio.2022.102881
Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants
- James Carothers
- November 15, 2022
- kedalionlabs.com
- Cholpisit Kiattisewee, Ava V Karanjia, Mateusz Legut, …, Neville E Sanjana, James M Carothers, Jesse G Zalatan
- ACS Synth Biol, 10.1021/acssynbio.2c00405
Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems
- James Carothers
- November 20, 2021
- kedalionlabs.com
- Benjamin I Tickman, Diego Alba Burbano, Venkata P Chavali, …, Vincent Noireaux, Jesse G Zalatan, James M Carothers
- Cell Syst, 10.1016/j.cels.2021.10.008
Complex dependence of CRISPR-Cas9 binding strength on guide RNA spacer lengths
- James Carothers
- June 8, 2021
- kedalionlabs.com
- Aset Khakimzhan, David Garenne, Benjamin Tickman, Jason Fontana, James Carothers, Vincent Noireaux
- Phys Biol, 10.1088/1478-3975/ac091e
Challenges and opportunities with CRISPR activation in bacteria for data-driven metabolic engineering
- James Carothers
- June 30, 2020
- kedalionlabs.com
- Jason Fontana, David Sparkman-Yager, Jesse G Zalatan, James M Carothers
- Curr Opin Biotechnol, 10.1016/j.copbio.2020.04.005
Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
- James Carothers
- April 3, 2020
- kedalionlabs.com
- Jason Fontana, Chen Dong, Cholpisit Kiattisewee, …, Benjamin I Tickman, James M Carothers, Jesse G Zalatan
- Nat Commun, 10.1038/s41467-020-15454-y
Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course
- James Carothers
- August 8, 2019
- kedalionlabs.com
- Jesus F Barajas, Maren Wehrs, Milton To, …, Jeffrey L Fortman, Steven W Singer, Constance B Bailey
- None, 10.1128/jmbe.v20i2.1723
Author Correction: Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria
- James Carothers
- October 17, 2018
- kedalionlabs.com
- Chen Dong, Jason Fontana, Anika Patel, James M Carothers, Jesse G Zalatan
- Nat Commun, 10.1038/s41467-018-06909-4
Regulated Expression of sgRNAs Tunes CRISPRi in E. coli
- James Carothers
- April 11, 2018
- kedalionlabs.com
- Jason Fontana, Chen Dong, Jennifer Y Ham, Jesse G Zalatan, James M Carothers
- Biotechnol J, 10.1002/biot.201800069