Channel: Cell biology cryo-EM

Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on cell samples cooled to cryogenic temperatures. Wikipedia (CC BY-SA 3.0)

    2023 Articles

    629 articles

    Seeing the wood for the trees

    • Olivia E R Smith, Olivia E R Smith, Olivia E R Smith, Olivia E R Smith, Olivia E R Smith, Tanmay A M Bharat
    • Nat Methods, 10.1038/s41592-022-01741-7
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    Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA

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    Visualizing everything, everywhere, all at once: Cryo-EM and the new field of structureomics

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    Towards in situ high-resolution imaging of viruses and macromolecular complexes using cryo-electron tomography

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    Convolutional networks for supervised mining of molecular patterns within cellular context

    • Irene de Teresa-Trueba, Sara K Goetz, Alexander Mattausch, …, Anna Kreshuk, Julia Mahamid, Judith B Zaugg
    • Nat Methods, 10.1038/s41592-022-01746-2
    Comment Section

    TomoTwin: generalized 3D localization of macromolecules in cryo-electron tomograms with structural data mining

    • Gavin Rice, Thorsten Wagner, Markus Stabrin, …, Oleg Sitsel, Daniel Prumbaum, Stefan Raunser
    • Nat Methods, 10.1038/s41592-023-01878-z
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    Computational Methods Toward Unbiased Pattern Mining and Structure Determination in Cryo-Electron Tomography Data

    • Hannah Hyun-Sook Kim, Mostofa Rafid Uddin, Min Xu, …, Mostofa Rafid Uddin, Min Xu, Yi-Wei Chang
    • J Mol Biol, 10.1016/j.jmb.2023.168068
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    HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography

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    Bringing Structure to Cell Biology with Cryo-Electron Tomography

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    ELI trifocal microscope: a precise system to prepare target cryo-lamellae for in situ cryo-ET study

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    2022 Articles

    59 articles

    Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach

    • Haresh Bhaskar, Dirk-Jan Kleinjan, Curran Oi, …, Susan J Rosser, Mathew H Horrocks, Lynne Regan
    • Protein Sci, 10.1002/pro.4558
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    Mechanism of IFT-A polymerization into trains for ciliary transport

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    Maximum-likelihood model fitting for quantitative analysis of SMLM data

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    Structure of a volume-regulated heteromeric LRRC8A/C channel

    • Sonja Rutz, Dawid Deneka, Antje Dittmann, …, Antje Dittmann, Marta Sawicka, Raimund Dutzler
    • Nat Struct Mol Biol, 10.1038/s41594-022-00899-0
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    Soft X-ray tomograms provide a structural basis for whole-cell modeling

    • Valentina Loconte, Jian-Hua Chen, Bieke Vanslembrouck, …, Gerry McDermott, Mark A Le Gros, Carolyn A Larabell
    • FASEB J, 10.1096/fj.202200253r
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    Algorithm for Modern Electron Microscopic Examination of the Golgi Complex

    • Alexander A Mironov, Galina V Beznoussenko, Alexander A Mironov, …, Galina V Beznoussenko, Alexander A Mironov, Galina V Beznoussenko
    • Methods Mol Biol, 10.1007/978-1-0716-2639-9_12
    Comment Section

    Lessons Learned from Responsive Molecular Dynamics Studies of the COVID-19 Virus

    • David J Hardy, Barry Isralewitz, John E Stone, …, Barry Isralewitz, John E Stone, Emad Tajkhorshid
    • Proceedings of UrgentHPC 2021 : the Third International Workshop on HPC for Urgent Decision Making, 10.1109/urgenthpc54802.2021.00006
    Comment Section

    MINFLUX imaging of a bacterial molecular machine at nanometer resolution

    • Alexander Carsten, Maren Rudolph, Tobias Weihs, …, Antonio Virgilio Failla, Manuel Wolters, Martin Aepfelbacher
    • Methods Appl Fluoresc, 10.1088/2050-6120/aca880
    Comment Section

    Fine-grained, nonlinear registration of live cell movies reveals spatiotemporal organization of diffuse molecular processes

    • Xuexia Jiang, Tadamoto Isogai, Joseph Chi, …, Tadamoto Isogai, Joseph Chi, Gaudenz Danuser
    • PLoS Comput Biol, 10.1371/journal.pcbi.1009667
    Comment Section

    Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu

    Comment Section