Channel: Evolution of CRISPR-Cas systems

Inspired by: Eugene Koonin

The evolution of CRISPR-Cas systems in cells.

2023 Articles

526 articles

Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector

  • Hongshen Zhao, Qinghua Dong, Hongshen Zhao, …, Hongshen Zhao, Qinghua Dong, Tao Jiang
  • Nat Microbiol, 10.1038/s41564-022-01316-4
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Structural characterization of the type I-B CRISPR Cas7 from Thermobaculum terrenum

  • Pil-Won Seo, Do-Heon Gu, Ji-Won Kim, …, Jun-Hong Kim, Suk-Youl Park, Jeong-Sun Kim
  • Biochim Biophys Acta Proteins Proteom, 10.1016/j.bbapap.2023.140900
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First full views of a CRISPR-guided system for gene insertion

  • Orsolya Barabas, Phoebe A Rice, Orsolya Barabas, …, Phoebe A Rice, Orsolya Barabas, Phoebe A Rice
  • Nature, 10.1038/d41586-022-04584-6
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Structural snapshots of R-loop formation by a type I-C CRISPR Cascade

  • Roisin E O’Brien, Jack P K Bravo, Delisa Ramos, …, Grace N Hibshman, Jacquelyn T Wright, David W Taylor
  • Mol Cell, 10.1016/j.molcel.2023.01.024
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Structural Basis for the Ribonuclease Activity of a Thermostable CRISPR-Cas13a from Thermoclostridium caenicola

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Optimized protocols for the characterization of Cas12a activities

  • Lindsie Martin, Saadi Rostami, Rakhi Rajan, …, Lindsie Martin, Saadi Rostami, Rakhi Rajan
  • Methods Enzymol, 10.1016/bs.mie.2022.08.048
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Programmable RNA detection with CRISPR-Cas12a

  • Santosh R Rananaware, Emma K Vesco, Grace M Shoemaker, …, Nicolas C Macaluso, Long T Nguyen, Piyush K Jain
  • bioRxiv : the preprint server for biology, 10.1101/2023.01.29.525716
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Non-canonical inhibition strategies and structural basis of anti-CRISPR proteins targeting type I CRISPR-Cas systems

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Cas12n nucleases, early evolutionary intermediates of type V CRISPR, comprise a distinct family of miniature genome editors

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Programmable RNA detection with CRISPR-Cas12a

  • Santosh R Rananaware, Emma K Vesco, Grace M Shoemaker, …, Nicolas C Macaluso, Long T Nguyen, Piyush K Jain
  • Nat Commun, 10.1038/s41467-023-41006-1
Comment Section

2022 Articles

31 articles

Discovery and characterization of novel type I-D CRISPR-guided transposons identified among diverse Tn7-like elements in cyanobacteria

  • Shan-Chi Hsieh, Joseph E Peters, Shan-Chi Hsieh, …, Joseph E Peters, Shan-Chi Hsieh, Joseph E Peters
  • Nucleic Acids Res, 10.1093/nar/gkac1216
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Improved genome editing by an engineered CRISPR-Cas12a

  • Enbo Ma, Kai Chen, Honglue Shi, …, Kaihong Zhou, Jinjuan Ye, Jennifer A Doudna
  • Nucleic Acids Res, 10.1093/nar/gkac1192
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Single Molecule FRET Analysis of CRISPR Cas9 Single Guide RNA Folding Dynamics

  • Ikenna C Okafor, Taekjip Ha, Ikenna C Okafor, …, Taekjip Ha, Ikenna C Okafor, Taekjip Ha
  • J Phys Chem B, 10.1021/acs.jpcb.2c05428
Comment Section

Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic

  • Anna Nemudraia, Artem Nemudryi, Murat Buyukyoruk, …, Kristin S Koutmou, Andrew Santiago-Frangos, Blake Wiedenheft
  • Nat Commun, 10.1038/s41467-022-35445-5
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Compact Cas9d and HEARO enzymes for genome editing discovered from uncultivated microbes

  • Daniela S Aliaga Goltsman, Lisa M Alexander, Jyun-Liang Lin, …, Cristina N Butterfield, Brian C Thomas, Christopher T Brown
  • Nat Commun, 10.1038/s41467-022-35257-7
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A bacterial Argonaute from Tepiditoga spiralis with the ability of RNA guided plasmid cleavage

  • Xiaochen Xie, Longyu Wang, Lixin Ma, …, Xiaochen Xie, Longyu Wang, Lixin Ma
  • Biochem Biophys Res Commun, 10.1016/j.bbrc.2022.11.097
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Miniature CRISPR-Cas12f1-Mediated Single-Nucleotide Microbial Genome Editing Using 3′-Truncated sgRNA

  • Ho Joung Lee, Hyun Ju Kim, Sang Jun Lee, …, Ho Joung Lee, Hyun Ju Kim, Sang Jun Lee
  • CRISPR J, 10.1089/crispr.2022.0071
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CRISPR-Cas13a system: A novel tool for molecular diagnostics

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Prokaryotic ncRNAs: Master regulators of gene expression

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CRISPR-Cas Controls Cryptic Prophages

  • Sooyeon Song, Ekaterina Semenova, Konstantin Severinov, …, Michael J Benedik, Toshinari Maeda, Thomas K Wood
  • Int J Mol Sci, 10.3390/ijms232416195
Comment Section