Multiplexed Guide RNA Expression Leads to Increased Mutation Frequency in Targeted Window Using a CRISPR-Guided Error-Prone DNA Polymerase in Saccharomyces cerevisiae

ACS synthetic biology

Michael Gossing, Angelo Limeta, Christos Skrekas, …, Andrew Davis, Verena Siewers, Florian David

• DOI: 10.1021/acssynbio.2c00689
• PMID: 37486342
• PMCID: PMC10443033