Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease

Applied and environmental microbiology

Guo-Chang Zhang, In Iok Kong, Heejin Kim, Jing-Jing Liu, Jamie H D Cate, Yong-Su Jin

• DOI: 10.1128/aem.02310-14
• PMID: 25281382
• PMCID: PMC4249234