Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform

Andrei Sakai, Christopher R Deich, Frank H T Nelissen, …, Wilhelm T S Huck, Katarzyna P Adamala, John I Glass

• DOI: 10.1093/synbio/ysac008
• PMID: 35774105
• PMCID: PMC9239315