Probing Selectivity and Creating Structural Diversity Through Hybrid Polyketide Synthases
- Aaron A Koch, Jennifer J Schmidt, Andrew N Lowell, …, Katherine M Coburn, Joseph A Chemler, David H Sherman
- Angew Chem Int Ed Engl, 10.1002/anie.202004991
Probing the Flexibility of an Iterative Modular Polyketide Synthase with Non-Native Substrates in Vitro
- Samuel C Curran, Andrew Hagen, Sean Poust, …, Christopher J Petzold, Leonard Katz, Jay D Keasling
- ACS Chem Biol, 10.1021/acschembio.8b00422
An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
- Christian A Siltanen, Russell H Cole, Sean Poust, …, Jeff Ubersax, Zev J Gartner, Adam R Abate
- Sci Rep, 10.1038/s41598-018-25577-4
Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells
- Russell H Cole, Shi-Yang Tang, Christian A Siltanen, …, Sean Poust, Zev J Gartner, Adam R Abate
- Proc Natl Acad Sci U S A, 10.1073/pnas.1704020114
Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid
- Andrew Hagen, Sean Poust, Tristan de Rond, …, Leonard Katz, Christopher J Petzold, Jay D Keasling
- ACS Synth Biol, 10.1021/acssynbio.5b00153
Mechanistic Analysis of an Engineered Enzyme that Catalyzes the Formose Reaction
- Sean Poust, James Piety, Arren Bar-Even, …, David Baker, Jay D Keasling, Justin B Siegel
- Chembiochem, 10.1002/cbic.201500228
Computational protein design enables a novel one-carbon assimilation pathway
- Justin B Siegel, Amanda Lee Smith, Sean Poust, …, Barry L Stoddard, Mary E Lidstrom, David Baker
- Proc Natl Acad Sci U S A, 10.1073/pnas.1500545112
Understanding the role of histidine in the GHSxG acyltransferase active site motif: evidence for histidine stabilization of the malonyl-enzyme intermediate
- Sean Poust, Isu Yoon, Paul D Adams, Leonard Katz, Christopher J Petzold, Jay D Keasling
- PLoS One, 10.1371/journal.pone.0109421
Narrowing the gap between the promise and reality of polyketide synthases as a synthetic biology platform
- Sean Poust, Andrew Hagen, Leonard Katz, Jay D Keasling
- Curr Opin Biotechnol, 10.1016/j.copbio.2014.04.011