Switching an active site helix in dihydrofolate reductase reveals limits to subdomain modularity
- Victor Y Zhao, João V Rodrigues, Elena R Lozovsky, Daniel L Hartl, Eugene I Shakhnovich
- Biophys J, 10.1016/j.bpj.2021.09.032
Development of antibacterial compounds that constrain evolutionary pathways to resistance
- Yanmin Zhang, Sourav Chowdhury, João V Rodrigues, …, Sourav Chowdhury, João V Rodrigues, Eugene Shakhnovich
- Elife, 10.7554/elife.64518
Adaptation to mutational inactivation of an essential gene converges to an accessible suboptimal fitness peak
- João V Rodrigues, Eugene I Shakhnovich
- Elife, 10.7554/elife.50509
Chimeric dihydrofolate reductases display properties of modularity and biophysical diversity
- João V Rodrigues, C Brandon Ogbunugafor, Daniel L Hartl, Eugene I Shakhnovich
- Protein Sci, 10.1002/pro.3646
Proteostasis Environment Shapes Higher-Order Epistasis Operating on Antibiotic Resistance
- Rafael F Guerrero, Samuel V Scarpino, João V Rodrigues, Daniel L Hartl, C Brandon Ogbunugafor
- Genetics, 10.1534/genetics.119.302138
Evolution on the Biophysical Fitness Landscape of an RNA Virus
- Assaf Rotem, Adrian W R Serohijos, Connie B Chang, …, Andrew B Feldman, David A Weitz, Eugene I Shakhnovich
- Mol Biol Evol, 10.1093/molbev/msy131
Differential Enzyme Flexibility Probed Using Solid-State Nanopores
- Rui Hu, João V Rodrigues, Pradeep Waduge, …, Eugene Shakhnovich, Qing Zhao, Meni Wanunu
- ACS Nano, 10.1021/acsnano.8b00734
Stability of the Influenza Virus Hemagglutinin Protein Correlates with Evolutionary Dynamics
- Eili Y Klein, Deena Blumenkrantz, Adrian Serohijos, …, Andrew P Lane, Andrew Feldman, Andrew Pekosz
- mSphere, 10.1128/mspheredirect.00554-17
Rational Design of Novel Allosteric Dihydrofolate Reductase Inhibitors Showing Antibacterial Effects on Drug-Resistant Escherichia coli Escape Variants
- Bharath Srinivasan, João V Rodrigues, Sam Tonddast-Navaei, Eugene Shakhnovich, Jeffrey Skolnick
- ACS Chem Biol, 10.1021/acschembio.7b00175
Biophysical principles predict fitness landscapes of drug resistance
- João V Rodrigues, Shimon Bershtein, Anna Li, Elena R Lozovsky, Daniel L Hartl, Eugene I Shakhnovich
- Proc Natl Acad Sci U S A, 10.1073/pnas.1601441113
Ethylmalonic encephalopathy ETHE1 R163W/R163Q mutations alter protein stability and redox properties of the iron centre
- Bárbara J Henriques, Tânia G Lucas, João V Rodrigues, …, Valeria Tiranti, Peter Bross, Cláudio M Gomes
- PLoS One, 10.1371/journal.pone.0107157